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Production of butanol and polyhydroxyalkanoate from industrial waste by Clostridium beijerinckii ASU10
Author(s) -
Hassan Elhagag Ahmed,
AbdAlla Mohamed Hemida,
Zohri AbdelNaser Ahmed,
Ragaey Marwa M.,
Ali Shimaa Mohamed
Publication year - 2019
Publication title -
international journal of energy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.808
H-Index - 95
eISSN - 1099-114X
pISSN - 0363-907X
DOI - 10.1002/er.4514
Subject(s) - clostridium beijerinckii , polyhydroxyalkanoates , glycerol , fermentation , chemistry , food science , butanol , biofuel , acetone , bioenergy , bioconversion , biomass (ecology) , ethanol , biochemistry , microbiology and biotechnology , bacteria , biology , agronomy , genetics
Summary This study demonstrated a biotechnological approach for simultaneous production of low‐cost H 2 , liquid biofuels, and polyhydroxyalkanoates (PHAs) by solventogenic bacterium ( Clostridium beijerinckii ) from renewable industrial wastes such as molasses and crude glycerol. C beijerinckii ASU10 (KF372577) exhibited considerable performance for hydrogen production of 5.1 ± 0.84 and 11 ± 0.44 mL H 2 h −1 on glycerol and sugarcane molasses, respectively. The total acetone‐butanol‐ethanol (ABE) generation from glycerol and molasses was 9.334 ± 2.98 and 10.831 ± 4.1 g L −1 , respectively. ABE productivity (g L −1 h −1 ) was 0.0486 and 0.0564 with a yield rate (g g −1 ) up to 0.508 and 0.493 from glycerol and molasses fermentation, respectively. The PHA yields from glycerol and sugarcane molasses were 84.37% and 37.97% of the dried bacterial biomass, respectively. Additionally, the ultrathin section of C beijerinckii ASU10 showed that PHA granules were accumulated more densely on glycerol than molasses. Gas chromatography–mass spectrometry (GC‐MS) analysis confirmed that the PHAs obtained from molasses fermentation included 3‐hydroxybutyrate (47.3%) and 3‐hydroxyoctanoate (52.7%) as the main constituents. Meanwhile, 3‐hydroxybutyrate represented the sole monomer of PHA produced from glycerol fermentation. This study demonstrated that C beijerinckii ASU10 (KF372577) is a potent strain for low‐cost PHA production depending on its high potential to produce high‐energy biofuel and other valuable compounds from utilization of organic waste materials.