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Enhancement of hydrogen production from palm oil mill effluent via cell immobilisation technique
Author(s) -
Singh Lakhveer,
Wahid Zularisam A.
Publication year - 2015
Publication title -
international journal of energy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.808
H-Index - 95
eISSN - 1099-114X
pISSN - 0363-907X
DOI - 10.1002/er.3231
Subject(s) - pome , biohydrogen , chemistry , hydrogen production , effluent , hydrogen , peg ratio , nuclear chemistry , polyethylene glycol , pulp and paper industry , hydrogen peroxide , chemical oxygen demand , chemical engineering , waste management , organic chemistry , wastewater , engineering , economics , finance
Summary Clostridium sp. LS2 was immobilised by entrapment in polyethylene glycol (PEG) gel beads to improve the biohydrogen production rate from palm oil mill effluent (POME). We sought to explore and optimise the hydrogen production capability of the immobilised cells by studying the conditions for cell immobilisation, including PEG concentration, cell loading and curing times, as well as the effects of temperature and K 2 HPO 4 (500–2000 mg/L), NiCl 2 (0.1–5.0 mg/L), FeCl 2 (100–400 mg/L) MgSO 4 (50–200 mg/L) concentrations on hydrogen production rate. The results showed that by optimising the PEG concentration (10% w/v), initial biomass (2.2 g dry weight), curing time (80 min) and temperature (37 °C), as well as the concentrations of K 2 HPO 4 (2000 mg/L), NiCl 2 (1 mg/L), FeCl 2 (300 mg/L) and MgSO 4 (100 mg/L), a maximum hydrogen production rate of 7.3 L/ l ‐POME/day and a yield of 0.31 L H 2 /g chemical oxygen demand were obtained during continuous operation. We believe that this process may be potentially expanded for sustained and large‐scale hydrogen production. Copyright © 2014 John Wiley & Sons, Ltd.