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Treatment of partially hydrolyzed polyacrylamide by mixed bacteria isolated from wastewater
Author(s) -
Dong Liang,
Su Fei,
Wang YongZhong
Publication year - 2020
Publication title -
environmental progress and sustainable energy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.495
H-Index - 66
eISSN - 1944-7450
pISSN - 1944-7442
DOI - 10.1002/ep.13445
Subject(s) - polyacrylamide , hydrolysis , wastewater , chemistry , chromatography , bacteria , strain (injury) , acinetobacter , sewage , nuclear chemistry , environmental engineering , biology , organic chemistry , biochemistry , polymer chemistry , environmental science , anatomy , genetics , antibiotics
In this work, a mixed strain which could degrade hydrolyzed polyacrylamide (HPAM) was constructed based on SS5 and SS6 isolated from two wastewater samples. SS5 is a globular gram‐negative bacterium, and SS6 is a rod‐shaped gram‐negative bacterium. Moreover, SS5 and SS6 were identified as Acinetobacter sp. and Pseudomonas sp., respectively. The removal performance of the mixed strain was optimized using single‐factor experiment and response surface methodology. The maximal HPAM removal efficiency 29.78% was observed at pH 7.5, 35°C, 170 rpm rotation speed, and inoculum loading 3% (v/v) for 7 days. The optimal concentrations of nutrients were 0.32 g/L glucose, 0.21 g/L NaNO 3 , 2.10 g/L KH 2 PO 4 ‐K 2 HPO 4 , and the HPAM removal efficiency reached 45.82%. Finally, the optimal HPAM concentration and the HPAM removal efficiency were 300 mg/L and 45.82%, respectively. The results indicate that the mixed strain was a potential candidate for dealing with oil‐ or gas‐field sewage with high hydrolyzed polyacrylamide concentration.