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Efficient biodegradation of malachite green by a newly isolated Klebsiella pneumoniae strain WA‐1
Author(s) -
Li Chunsheng,
Liu Dandan,
Yang Xianqing,
Chen Shengjun,
Deng Jianchao,
Rong Hui,
Li Chi
Publication year - 2019
Publication title -
environmental progress and sustainable energy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.495
H-Index - 66
eISSN - 1944-7450
pISSN - 1944-7442
DOI - 10.1002/ep.13346
Subject(s) - biodegradation , malachite green , klebsiella pneumoniae , strain (injury) , chemistry , metal ions in aqueous solution , microorganism , chromatography , bacteria , nuclear chemistry , environmental chemistry , microbiology and biotechnology , metal , organic chemistry , biology , biochemistry , genetics , escherichia coli , adsorption , anatomy , gene
Biodegradation by microorganisms is a potential treatment technique of malachite green (MG) in aquaculture environments. In this study, a bacterium with high MG decolorization and biodegradation ability was isolated through enrichment culture and color‐decoloration circle method, and was identified as Klebsiella pneumoniae WA‐1 after molecular and biochemical identification. The MG biodegradation characteristics and the factors affecting the MG biodegradation ability of this strain were studied using KBH 4 reduction‐high performance liquid chromatography with fluorescence detector (HPLC‐FLD) method. K. pneumoniae WA‐1 could efficiently biodegrade MG in aqueous solution without any nutrients that over 94.0% MG was biodegraded by this strain at 1–20 mg/L within 0.5 hr and 100% MG were biodegraded at 1–10 mg/L within 12 hr. Fairly stable MG biodegradation performance was observed in complex environments, including various pH, temperature, salinity, and metal ions, especially at low MG concentration. Furthermore, the presence of NaCl, Mn 2+ , Mg 2+ , or Cu 2+ could enhance the MG biodegradation ability of this strain. These results indicate that K. pneumoniae WA‐1 could be used as a potential MG degradation agent in complex aquaculture environments.