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Bioethanol production by newly isolated halotolerant Kluyveromyces marxianus strains
Author(s) -
Ertuğrul Karatay Sevgi,
Demiray Ekin,
Dönmez Gönül
Publication year - 2018
Publication title -
environmental progress and sustainable energy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.495
H-Index - 66
eISSN - 1944-7450
pISSN - 1944-7442
DOI - 10.1002/ep.12935
Subject(s) - kluyveromyces marxianus , halotolerance , fermentation , biofuel , ethanol fuel , food science , ethanol , chemistry , yeast , bioconversion , yield (engineering) , salinity , botany , microbiology and biotechnology , biology , biochemistry , materials science , saccharomyces cerevisiae , ecology , metallurgy
Several biotechnological applications have been investigated using Kluyveromyces spp. yeast, considering their high metabolic diversity. This study suggests the usage of two newly isolated halotolerant K. marxianus strains obtained from cheese and pickle wastes could be used for bioethanol production in molasses medium. Some important parameters such as pH (4, 5, 6, 7), fermentation period (4, 8, 12, 24 h), initial substrate loading (6, 8, 10, 12, 14 v/v %), and salt concentrations (2, 4, 6, 8, 10 w/v %) were optimized in the first experiments of the study, which showed that the optimum medium was the one containing 10 v/v % molasses solution and 4 w/v % NaCl. The highest ethanol yield was obtained after 12 h of fermentation at pH 5 in the first isolate and at pH 6 in the second one. Moreover 7.90 and 7.92 g/L of ethanol, corresponding to 0.65 g/L.h of productivity and 39.2% and 39.1% theoretical ethanol yield achieved respectively. It is also worth to mention here that considerable ethanol quantities such as 2.84 and 2.65 g/L were obtained even at 10% salt concentration. To the best of our knowledge this is the first report on usage of halotolerant K. marxianus in molasses‐based ethanol production. © 2018 American Institute of Chemical Engineers Environ Prog, 38: 542–547, 2019