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Assessment of P leurotus ostreatus mediated degradation of agro‐residues by using design of experiments methodologies
Author(s) -
Papadopoulou Konstantina,
Dimitropoulos Vassilis,
Rigas Fotis
Publication year - 2015
Publication title -
environmental progress and sustainable energy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.495
H-Index - 66
eISSN - 1944-7450
pISSN - 1944-7442
DOI - 10.1002/ep.12176
Subject(s) - pleurotus ostreatus , lignin , straw , central composite design , cellulose , pulp and paper industry , lignocellulosic biomass , pleurotus , decomposer , factorial experiment , microbiology and biotechnology , agricultural waste , residue (chemistry) , agronomy , food science , response surface methodology , chemistry , mathematics , biology , botany , waste management , engineering , mushroom , chromatography , ecology , biochemistry , statistics , organic chemistry , ecosystem
Aim Lignocellulosic biomass, mostly by agricultural and forestry sources, mainly consists of cellulose, hemicelluloses, and lignin. White rot fungi are known to be efficient degraders of cellulose due to their ligninolytic enzyme system. The use of Pleurotus ostreatus AMRL 141 as a biological treatment of wheat straw (WS) was explored to determine the most significant variables affecting the cultivation of fungus. Methods Two experimental designs were applied: firstly, a 2‐level factorial design (screening) with 7 variables and 3 responses and a central composite design with 4 variables and 3 responses. The optima of the statistically adequate models obtained for a 2 weeks cultivation period were then found. The solid state medium used was sandy soil mixed with agricultural residue (milled WS). Results Overall, the results of many previous studies of our research group have proved that Pleurotus ostreatus is an efficient lignocellulosic decomposer. In this study, design of experiments methodologies were successfully used and the optimum values of responses found for the two weeks cultivation period were lag time (2 days), extent of colonization (46 mm), and propagation velocity (3 mm d −1 ). © 2015 American Institute of Chemical Engineers Environ Prog, 34: 1705–1713, 2015