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Simplification and optimization of media ingredients for enhanced production of CMCase by newly isolated Bacillus subtilis NA15
Author(s) -
Akhtar Nadeem,
Aanchal,
Goyal Dinesh,
Goyal Arun
Publication year - 2014
Publication title -
environmental progress and sustainable energy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.495
H-Index - 66
eISSN - 1944-7450
pISSN - 1944-7442
DOI - 10.1002/ep.12004
Subject(s) - bacillus subtilis , cellulase , 16s ribosomal rna , yeast extract , food science , strain (injury) , response surface methodology , ribosomal rna , cellulose , bacteria , chemistry , biology , microbiology and biotechnology , gene , biochemistry , fermentation , chromatography , genetics , anatomy
Cellulose degrading bacterium was isolated from composting biomass and identified as Bacillus subtilis on the basis of phenotypic fingerprint using GEN III MicroPlate TM and 16S ribosomal RNA (rRNA) gene sequence analysis. 16S rRNA gene sequence similarity analysis showed 99% similarity with Bacillus subtilis. The screening of 11 nutrients was done using Plackett‐Burman design to explicate the parameters that significantly influence the carboxy methyl cellulase (CMCase) production. Maximum CMCase production of 0.47 U/mL was obtained using response surface methodology with carboxy methyl cellulose (CMC): 18 g/L, peptone: 5 g/L, yeast extract: 5 g/L and MnCl 2 : 0.5 g/L. The model predicted the maximum CMCase activity (0.45 U/mL), which was in good agreement with the experimental value of 0.47 U/mL showing sevenfold increase as compared to unoptimized medium. Presence of MnCl 2 in the medium significantly enhanced the CMCase production. © 2014 American Institute of Chemical Engineers Environ Prog, 34: 533–541, 2015