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Efficient pretreatment for bioethanol production from water hyacinth ( eichhornia crassipes ) involving naturally isolated and recombinant enzymes and its recovery
Author(s) -
Das Saprativ P.,
Ravindran Rajeev,
Ghosh Arabinda,
Deka Deepmoni,
Das Debasish,
Jawed Mohammad,
Fontes Carlos M.G.A.,
Goyal Arun
Publication year - 2014
Publication title -
environmental progress and sustainable energy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.495
H-Index - 66
eISSN - 1944-7450
pISSN - 1944-7442
DOI - 10.1002/ep.11885
Subject(s) - cellulase , fermentation , chemistry , ethanol fuel , eichhornia crassipes , trichoderma reesei , hydrolysis , food science , hemicellulose , clostridium thermocellum , biochemistry , biology , aquatic plant , ecology , macrophyte
Simultaneous saccharification and fermentation (SSF) experiments involving water hyacinth ( Eichhornia crassipes ), an abundantly available renewable bioresource, using hydrolytic enzymes, and fermentative microbes were investigated. Water hyacinth containing 30.01 (%, w/w) cellulose, 44.49 (%, w/w) hemicellulose, and 20.04 (%, w/w) lignin was subjected to three different pretreatments, namely, wet oxidation, phosphoric acid (H 3 PO 4 )‐acetone, and ammonia fiber explosion (AFEX). Hydrolytic enzymes, namely, recombinant Clostridium thermocellum cellulase (GH5) and hemicellulase (GH43), Trichoderma reesei and Bacillus subtilis AS3 cellulases were employed separately for saccharification. Saccharomyces cerevisiae and Candida shehatae were used for fermentation. The AFEX pretreated 1% (w/v) water hyacinth along with recombinant cellulase (GH5)‐hemicellulase (GH43) consortium gave the highest ethanol titer of 1.52 g/L as compared with wet oxidation (1.23 g/L) and phosphoric acid‐acetone pretreatments (1.31 g/L). The best SSF combination with 5% (w/v) substrate at shake flask contributed an ethanol titer and yield of 7.83 g/L, 0.266 (g of ethanol/g of substrate) and its scale up at bioreactor level resulted in significantly higher ethanol titer and yield of 14.39 g/L and 0.489 (g/g), respectively. 93.0 (%, v/v) ethanol from bioreactor was recovered by rotary evaporator with 20.4% purification efficiency. © 2013 American Institute of Chemical Engineers Environ Prog, 33: 1396–1404, 2014