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Characterization of cellulase producing Bacillus sp. for effective degradation of leaf litter biomass
Author(s) -
Akhtar Nadeem,
Sharma Ashish,
Deka Deepmoni,
Jawed M.,
Goyal Dinesh,
Goyal Arun
Publication year - 2013
Publication title -
environmental progress and sustainable energy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.495
H-Index - 66
eISSN - 1944-7450
pISSN - 1944-7442
DOI - 10.1002/ep.11726
Subject(s) - cellulase , bacillus megaterium , bacillus subtilis , bacillus (shape) , food science , 16s ribosomal rna , carboxymethyl cellulose , biology , bacillales , cellulose , botany , microbiology and biotechnology , chemistry , bacteria , biochemistry , sodium , organic chemistry , genetics
Cellulose degrading Bacillus spp. (AS1, AS2, and AS3) were isolated from cow dung and identified phylogenetically by 16S ribosomal RNA (16S rRNA) sequence analysis. Bacillus sp. AS1 showed 99% homology with Bacillus megaterium whereas, Bacillus sp. AS2 and AS3 were similar to B. subtilis. The activity of extracellular carboxymethyl cellulase in presence of 1% carboxymethyl cellulose at 50°C was 0.04 U/mL for Bacillus sp. AS1 and 0.06 U/mL for Bacillus sp. AS2 at pH 7.0, whereas higher activity of 0.08 U/mL was observed for Bacillus sp. AS3 at pH 9.0. Crude cellulase from all the three Bacillus sp. were stable over a wide range of pH (5.0–9.0) and thermally stable up to 60°C. Carboxymethyl cellulase, filter paperase, and β‐glucosidase activity of Bacillus sp. AS3 was 0.07, 0.02, and 0.04 U/ml, respectively. Degradation of leaf litter biomass treated by Bacillus sp. AS3 showed 47% drop in carbon:nitrogen (C:N) ratio and x‐ray diffractograph revealed decline in crystalline structure. © 2012 American Institute of Chemical Engineers Environ Prog, 32: 1195–1201, 2013
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