Open AccessToll‐like receptor 7 stimulates production of specialized pro‐resolving lipid mediators and promotes resolution of airway inflammation
Author(s) -
Koltsida Ourania,
Karamnov Sergey,
Pyrillou Katerina,
Vickery Thad,
Chairakaki AikateriniDimitra,
Tamvakopoulos Constantin,
Sideras Paschalis,
Serhan Charles N.,
Andreakos Evangelos
Publication year - 2013
Publication title -
embo molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.923
H-Index - 107
eISSN - 1757-4684
pISSN - 1757-4676
DOI - 10.1002/emmm.201201891
Subject(s) - tlr7 , inflammation , docosahexaenoic acid , lipid signaling , receptor , immunology , toll like receptor , biology , chemistry , polyunsaturated fatty acid , immune system , innate immune system , biochemistry , fatty acid
Although specialized pro‐resolving mediators (SPMs) biosynthesized from polyunsaturated fatty acids are critical for the resolution of acute inflammation, the molecules and pathways that induce their production remain elusive. Here, we show that TLR7, a receptor recognizing viral ssRNA and damaged self‐RNA, mobilizes the docosahexaenoic acid (DHA)‐derived biosynthetic pathways that lead to the generation of D‐series SPMs. In mouse macrophages and human monocytes, TLR7 activation triggered production of DHA‐derived monohydroxy metabolome markers and generation of protectin D1 (PD1) and resolvin D1 (RvD1). In mouse allergic airway inflammation, TLR7 activation enhanced production of DHA‐derived SPMs including PD1 and accelerated the catabasis of Th2‐mediated inflammation. D‐series SPMs were critical for TLR7‐mediated resolution of airway inflammation as this effect was lost in Alox15 −/− mice, while resolution was enhanced after local administration of PD1 or RvD1. Together, our findings reveal a new previously unsuspected role of TLR7 in the generation of D‐series SPMs and the resolution of allergic airway inflammation. They also identify TLR stimulation as a new approach to drive SPMs and resolution of inflammatory diseases.