Open AccessHaematopoietic stem cell differentiation promotes the release of prominin‐1/CD133‐containing membrane vesicles—a role of the endocytic–exocytic pathway
Author(s) -
Bauer Nicola,
WilschBräuninger Michaela,
Karbanová Jana,
Fonseca AnaVioleta,
Strauss Doreen,
Freund Daniel,
Thiele Christoph,
Huttner Wieland B.,
Bornhäuser Martin,
Corbeil Denis
Publication year - 2011
Publication title -
embo molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.923
H-Index - 107
eISSN - 1757-4684
pISSN - 1757-4676
DOI - 10.1002/emmm.201100147
Subject(s) - microbiology and biotechnology , stem cell , progenitor cell , biology , cellular differentiation , haematopoiesis , endocytic cycle , lipid raft , microvesicles , stromal cell , cell , endocytosis , signal transduction , biochemistry , cancer research , microrna , gene
Abstract The differentiation of stem cells is a fundamental process in cell biology and understanding its mechanism might open a new avenue for therapeutic strategies. Using an ex vivo co‐culture system consisting of human primary haematopoietic stem and progenitor cells growing on multipotent mesenchymal stromal cells as a feeder cell layer, we describe here the exosome‐mediated release of small membrane vesicles containing the stem and cancer stem cell marker prominin‐1 (CD133) during haematopoietic cell differentiation. Surprisingly, this contrasts with the budding mechanism underlying the release of this cholesterol‐binding protein from plasma membrane protrusions of neural progenitors. Nevertheless, in both progenitor cell types, protein–lipid assemblies might be the essential structural determinant in the release process of prominin‐1. Collectively, these data support the concept that prominin‐1‐containing lipid rafts may host key determinants necessary to maintain stem cell properties and their quantitative reduction or loss may result in cellular differentiation.