Open AccessQuantitative tracking of T cell clones after haematopoietic stem cell transplantation
Author(s) -
Mamedov Ilgar Z.,
Britanova Olga V.,
Bolotin Dmitriy A.,
Chkalina Anna V.,
Staroverov Dmitriy B.,
Zvyagin Ivan V.,
Kotlobay Alexey A.,
Turchaninova Maria A.,
Fedorenko Denis A.,
Novik Andrew A.,
Sharonov George V.,
Lukyanov Sergey,
Chudakov Dmitriy M.,
Lebedev Yuri B.
Publication year - 2011
Publication title -
embo molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.923
H-Index - 107
eISSN - 1757-4684
pISSN - 1757-4676
DOI - 10.1002/emmm.201100129
Subject(s) - transplantation , haematopoiesis , biology , stem cell , immune system , immunology , cell , hematopoietic stem cell transplantation , cancer research , microbiology and biotechnology , genetics , medicine
Autologous haematopoietic stem cell transplantation is highly efficient for the treatment of systemic autoimmune diseases, but its consequences for the immune system remain poorly understood. Here, we describe an optimized RNA‐based technology for unbiased amplification of T cell receptor beta‐chain libraries and use it to perform the first detailed, quantitative tracking of T cell clones during 10 months after transplantation. We show that multiple clones survive the procedure, contribute to the immune response to activated infections, and form a new skewed and stable T cell receptor repertoire.