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Genome‐wide screen identifies signaling pathways that regulate autophagy during Caenorhabditis elegans development
Author(s) -
Guo Bin,
Huang Xinxin,
Zhang Peipei,
Qi Linxiang,
Liang Qianqian,
Zhang Xuebo,
Huang Jie,
Fang Bin,
Hou Wenru,
Han Jinghua,
Zhang Hong
Publication year - 2014
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1002/embr.201338310
Subject(s) - autophagy , biology , caenorhabditis elegans , microbiology and biotechnology , signal transduction , tfeb , bag3 , tor signaling , multicellular organism , model organism , gene , genetics , apoptosis
The mechanisms that coordinate the regulation of autophagy with developmental signaling during multicellular organism development remain largely unknown. Here, we show that impaired function of ribosomal protein RPL ‐43 causes an accumulation of SQST ‐1 aggregates in the larval intestine, which are removed upon autophagy induction. Using this model to screen for autophagy regulators, we identify 139 genes that promote autophagy activity upon inactivation. Various signaling pathways, including Sma/Mab TGF ‐β signaling, lin‐35 /Rb signaling, the XBP ‐1‐mediated ER stress response, and the ATFS ‐1‐mediated mitochondrial stress response, regulate the expression of autophagy genes independently of the TFEB homolog HLH ‐30. Our study thus provides a framework for understanding the role of signaling pathways in regulating autophagy under physiological conditions.