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The presequence pathway is involved in protein sorting to the mitochondrial outer membrane
Author(s) -
Wenz LenaSophie,
Opaliński Łukasz,
Schuler MaxHinderk,
Ellenrieder Lars,
Ieva Raffaele,
Böttinger Lena,
Qiu Jian,
Laan Martin,
Wiedemann Nils,
Guiard Bernard,
Pfanner Nikolaus,
Becker Thomas
Publication year - 2014
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1002/embr.201338144
Subject(s) - translocase of the inner membrane , translocase of the outer membrane , bacterial outer membrane , protein targeting , microbiology and biotechnology , mitochondrial carrier , mitochondrial membrane transport protein , mitochondrion , transport protein , biology , membrane protein , inner mitochondrial membrane , chemistry , biochemistry , membrane , gene , escherichia coli
The mitochondrial outer membrane contains integral α‐helical and β‐barrel proteins that are imported from the cytosol. The machineries importing β‐barrel proteins have been identified, however, different views exist on the import of α‐helical proteins. It has been reported that the biogenesis of Om45, the most abundant signal‐anchored protein, does not depend on proteinaceous components, but involves direct insertion into the outer membrane. We show that import of Om45 occurs via the translocase of the outer membrane and the presequence translocase of the inner membrane. Assembly of Om45 in the outer membrane involves the MIM machinery. Om45 thus follows a new mitochondrial biogenesis pathway that uses elements of the presequence import pathway to direct a protein to the outer membrane.