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FIP 5 phosphorylation during mitosis regulates apical trafficking and lumenogenesis
Author(s) -
Li Dongying,
Mangan Anthony,
Cicchini Louis,
Margolis Ben,
Prekeris Rytis
Publication year - 2014
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1002/embr.201338128
Subject(s) - mitosis , microbiology and biotechnology , phosphorylation , biology , chemistry
Apical lumen formation is a key step during epithelial morphogenesis. The establishment of the apical lumen is a complex process that involves coordinated changes in plasma membrane composition, endocytic transport, and cytoskeleton organization. These changes are accomplished, at least in part, by the targeting and fusion of Rab11/ FIP 5‐containing apical endosomes with the apical membrane initiation site ( AMIS ). Although AMIS formation and polarized transport of Rab11/ FIP 5‐containing endosomes are crucial for the formation of a single apical lumen, the spatiotemporal regulation of this process remains poorly understood. Here, we demonstrate that the formation of the midbody during cytokinesis is a symmetry‐breaking event that establishes the location of the AMIS . The interaction of FIP 5 with SNX 18, which is required for the formation of apical endocytic carriers, is inhibited by GSK ‐3 phosphorylation at FIP 5‐T276. Importantly, we show that FIP 5‐T276 phosphorylation occurs specifically during metaphase and anaphase, to ensure the fidelity and timing of FIP 5‐endosome targeting to the AMIS during apical lumen formation.