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Endosomal transport of septin mRNA and protein indicates local translation on endosomes and is required for correct septin filamentation
Author(s) -
Baumann Sebastian,
König Julian,
Koepke Janine,
Feldbrügge Michael
Publication year - 2014
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1002/embr.201338037
Subject(s) - septin , filamentation , endosome , translation (biology) , microbiology and biotechnology , messenger rna , intracellular transport , biology , chemistry , cytokinesis , gene , genetics , intracellular , cell , cell division , physics , laser , optics
Endosomes transport lipids and proteins over long distances by shuttling along microtubules. They also carry mRNA s on their surface, but the precise molecular function of this trafficking process is unknown. By live cell imaging of polarized fungal hyphae, we show microtubule‐dependent transport of septin mRNA and encoded septin protein on the same shuttling endosomes. Consistent with the hypothesis that septin mRNA is translated on endosomes, the accumulation of septin protein on endosomes requires the recruitment of septin mRNA . Furthermore, ribosomal proteins co‐localise with shuttling endosomes, but only if mRNA is present. Importantly, endosomal trafficking is essential for an efficient delivery of septin protein to filaments at growth poles, a process necessary to establish unipolar growth. Thus, we propose that local mRNA translation loads endosomes with septins for assembly and efficient delivery to septin filaments.