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The cohesin acetyltransferase Eco1 coordinates rDNA replication and transcription
Author(s) -
Lu Shuai,
Lee Kenneth K,
Harris Bethany,
Xiong Bo,
Bose Tania,
Saraf Anita,
Hattem Gaye,
Florens Laurence,
Seidel Chris,
Gerton Jennifer L
Publication year - 2014
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1002/embr.201337974
Subject(s) - cohesin , biology , transcription (linguistics) , genetics , microbiology and biotechnology , acetyltransferase , gene , meiosis , acetylation , linguistics , philosophy
Eco1 is the acetyltransferase that establishes sister‐chromatid cohesion during DNA replication. A budding yeast strain with an eco1 mutation that genocopies Roberts syndrome has reduced ribosomal DNA (rDNA) transcription and a transcriptional signature of starvation. We show that deleting FOB1—a gene that encodes a replication fork‐blocking protein specific for the rDNA region—rescues rRNA production and partially rescues transcription genome‐wide. Further studies show that deletion of FOB1 corrects the genome‐wide replication defects, nucleolar structure, and rDNA segregation that occur in the eco1 mutant. Our study highlights that the presence of cohesin at the rDNA locus has a central role in controlling global DNA replication and gene expression.