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Gene expression: degrade to derepress
Author(s) -
McShane Erik,
Selbach Matthias
Publication year - 2014
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/embj.201387752
Subject(s) - proteome , biology , gene , repressor , transcription (linguistics) , proteasome , chromatin immunoprecipitation , genetics , computational biology , gene expression , promoter , philosophy , linguistics
Chromatin immunoprecipitation and sequencing ( C h IP ‐seq) provides a static snap‐shot of DNA ‐associated proteins which fails to reflect the dynamics of the DNA ‐bound proteome. Now, C atic and co‐workers combine ubiquitin C h IP ‐seq and proteasome inhibitors to map sites of DNA ‐associated protein degradation on a genome‐wide scale. They identify an ubiquitin ligase which targets a transcriptional repressor for destruction by the proteasome, thus activating transcription of specific genes. These findings reveal that the ubiquitin proteasome system actively regulates transcription.