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Regulation of a transcription factor network by C dk1 coordinates late cell cycle gene expression
Author(s) -
Landry Benjamin D,
Mapa Claudine E,
Arsenault Heather E,
Poti Kristin E,
Benanti Jennifer A
Publication year - 2014
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/embj.201386877
Subject(s) - biology , transcription factor , gene expression , genetics , regulation of gene expression , gene , microbiology and biotechnology , cell cycle , cyclin dependent kinase 1 , expression (computer science) , transcription (linguistics) , computational biology , linguistics , philosophy , computer science , programming language
Abstract To maintain genome stability, regulators of chromosome segregation must be expressed in coordination with mitotic events. Expression of these late cell cycle genes is regulated by cyclin‐dependent kinase ( C dk1), which phosphorylates a network of conserved transcription factors ( TF s). However, the effects of C dk1 phosphorylation on many key TF s are not known. We find that elimination of C dk1‐mediated phosphorylation of four S ‐phase TF s decreases expression of many late cell cycle genes, delays mitotic progression, and reduces fitness in budding yeast. Blocking phosphorylation impairs degradation of all four TF s. Consequently, phosphorylation‐deficient mutants of the repressors Y ox1 and Y hp1 exhibit increased promoter occupancy and decreased expression of their target genes. Interestingly, although phosphorylation of the transcriptional activator H cm1 on its N ‐terminus promotes its degradation, phosphorylation on its C ‐terminus is required for its activity, indicating that C dk1 both activates and inhibits a single TF . We conclude that C dk1 promotes gene expression by both activating transcriptional activators and inactivating transcriptional repressors. Furthermore, our data suggest that coordinated regulation of the TF network by C dk1 is necessary for faithful cell division.