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Methylglyoxal induces hprt mutation and DNA adducts in human T‐lymphocytes in vitro
Author(s) -
Hou SaiMei,
Nori Peri,
Fang JiaLong,
Vaca Carlos E.
Publication year - 1995
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850260404
Subject(s) - methylglyoxal , hypoxanthine guanine phosphoribosyltransferase , in vitro , mutation , dna , genetics , biology , dna damage , mutagen , microbiology and biotechnology , biochemistry , chemistry , mutant , gene , enzyme
Methylglyoxal (MG) is a mutagen present in several foodstuffs, including coffee. We have used the 32 P‐postlabelling method to measure MG‐deoxyguanosine adduct levels, and the T‐cell cloning technique, to study the frequency of hprt (hypoxanthine‐guanine phosphoribosyl transferase) mutant cells after treatment of human lymphocytes with MG in vitro. The mutant induction by single (18 hr) high‐dose (1.0–1.5 mM) treatment was comparable to that induced by repeated (3 × 48 hr) low‐dose (0.1–0.4 mM) treatment. The latter also correlated with the adduct levels measured in the same experiment. The relative cell survival measured by direct cloning after the final treatment agreed well with the growth curves monitored during the expression phase. Our results show that MG is capable of inducing hprt mutations as well as DNA adducts in human lymphocytes at doses with low cytotoxicity. However, significant adduct formation (two‐ to threefold) could be obtained only after the first exposure in cells subjected to a repeated treatment protocol, and the induced mutant frequency was low (two‐to fourfold over background). Thus, MG seems to be a comparatively weak mutagen in this system. © 1995 Wiley‐Liss, Inc.