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Increased frequency of mutations at A:T base pairs in the bone marrow of B6C3F1 lacl transgenic mice exposed to 1, 3‐butadiene
Author(s) -
Recio Leslie,
Meyer Kathy G.
Publication year - 1995
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850260102
Subject(s) - point mutation , mutation frequency , mutation , microbiology and biotechnology , bone marrow , biology , carcinogenesis , transgene , genetics , immunology , gene
We have examined the spectra of mutations in a collection of 74 lacl mutants isolated from the bone marrow of B6C3F1 lacl transgenic mice exposed to 1, 250 ppm 1, 3‐butadiene (BD). Of the 49 independent mutations analyzed in the present study, 30 of 49 (61%) were point mutations at G:C base pairs, and 10 of 49 (20%) were point mutations at A:T base pairs. The remaining mutations consisted of small deletions and insertions and a single tandem change. An analysis of variance (ANOVA) on the frequency of specific mutations observed in each animal in this mutagenicity experiment [present study; also Sisk SC et al. (1994): Carcinogenesis 15:471–477] indicated that the frequency of point mutations at A:T base pairs is significantlantly greater ( P < 0.05) in BD‐exposed mice than in the air controls. In addition, there was a decrease ( P < 0.05; Fisher's exact test) in the frequency of G:C→A:T transitions at 5′‐CpG‐3′ sites in BD‐exposed mice (27%) relative to air controls (51%). These data indicate that subchronic exposures to BD induces an increased frequency of in vivo mutation at A:T base pairs in the bone marrow of B6C3F1 lacl transgenic mice. © 1995 Wiley‐Liss, Inc.