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Induction of polyploidy in human lymphocytes in vitro by excess adenine, but not by adenosine
Author(s) -
Edwards Anne J.,
Anderson Diana,
Phillips B. J.
Publication year - 1995
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850250305
Subject(s) - endoreduplication , adenosine , sister chromatid exchange , sister chromatids , polyploid , biology , clastogen , genetics , in vitro , microbiology and biotechnology , mitosis , dna , chromatid , in vivo , biochemistry , deoxyadenosine , cell , ploidy , cell cycle , micronucleus test , toxicity , chromosome , medicine , gene
It is known that high levels of DNA precursors can be both clastogenic and mutagenic in cultured cell lines and in vivo. The purpose of the present study was to examine at an observational level the cytogenetic effects of adenine and adenosine in primary human cell cultures. Human peripheral blood lymphocytes from four donors were cultured and treated with a range of concentrations of adenine and adenosine. Although no increase in sister chromatid exchange (SCE) frequency was observed with either compound, there was a statistically significant, dose‐related increase in the proportion of polyploid cells in cultures treated with adenine, but not in those treated with adenosine. Some of the polyploid metaphases found after adenine treatment contained diplochromosomes, suggesting that endoreduplication might have been involved in polyploid formation in these cells. It is concluded that a high level of adenine can cause genetic changes in human lymphocytes by interfering with mitosis, perhaps by disturbing the balance of DNA precursor pools. © 1995 Wiley‐liss, Inc.