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Effects of spermine on formation of HGPRT − mutants induced by ethylmethanesulfonate, methylmethanesulfonate, and mitomycin C in V79 chinese hamster cells
Author(s) -
Fiorio R.,
Vellosi R.,
Bronzetti G.
Publication year - 1994
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850230406
Subject(s) - spermine , chinese hamster , mutant , mitomycin c , hamster , polyamine , mutation , biology , chemistry , microbiology and biotechnology , biochemistry , dna , genetics , gene , enzyme
Spermine is a polyamine found in bacteria, animal, and plant tissues. It is involved in a variety of biological processes, and its interaction with DMA stabilizes the secondary structure of the double helix. Spermine is one of the first reported antimutagens, reducing the mutation rate in several prokaryotic test systems, while in eukaryotic organisms conflicting results have been obtained. In light of the significant antimutagenic effect of spermine, it is important to evaluate its activity in mammalian cells in culture. The present study was undertaken to evaluate the ability of spermine to suppress the level of HGPRT mutants induced by ethylmethanesulfonate, methylmethanesulfonate, and mitomycin C. Spermine reduced the mutation frequency induced by ethylmethanesulfonate and methylmethanesulfonate but did not affect survival; with mitomycin C survival was reduced but mutation rate was not influenced.