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Mutability of salmonella tester strains TA1538 ( hisD3052 ) and TA1535 ( hisG46 ) containing the UmuD' and UmuC proteins of escherichia coli
Author(s) -
Urios Amparo,
Herrera Guadalupe,
Aleixandre Vicente,
Sommer Suzanne,
Blanco Manuel
Publication year - 1994
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850230404
Subject(s) - reversion , escherichia coli , frameshift mutation , salmonella , plasmid , operon , mutant , chemistry , genetics , ames test , sos response , mutagen , biology , enterobacteriaceae , microbiology and biotechnology , mutation , bacteria , dna , gene , phenotype
We have studied the mutability of Salmonella typhimurium tester strains carrying plasmids in which either the umuDC or the umuD'C operon of Escherichia coli have been cloned. Reversion of the hisD3052 frameshift mutation by benzo[a]pyrene (B[a]P), aflatoxin B1 (AFB1) and 1‐nitropyrene (1‐NP), was very efficiently promoted by UmuD' (the activated form of UmuD) and UmuC proteins. In contrast, UmuD'C proteins promoted a moderate reversion of the missense hisG46 allele by B[a]P, and were not effective in mediating this reversion by AFB1. The Salmonella tester strain carrying the hisD3052 allele and containing the E. coli UmuD'C proteins has a sensitivity toward frameshift mutagens similar to that of the MucAB containing strain TA98, and may be useful for obtaining a high level of mutants generated by the SOS mutagenic mechanism in the absence of MucAB proteins. © 1994 Wiley‐Liss, Inc.