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Cytogenetic effects of phosphine inhalation by rodents. I: Acute 6‐hour exposure of mice
Author(s) -
Kligerman A. D.,
Bryant M. F.,
Doerr C. L.,
Erexson G. L.,
Kwanyuen P.,
McGee J. K.
Publication year - 1994
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850230306
Subject(s) - inhalation , inhalation exposure , toxicology , phosphine , acute exposure , carcinogen , biology , pharmacology , chemistry , physiology , medicine , genetics , anesthesia , biochemistry , endocrinology , catalysis
Phosphine (PH 3 ) is a highly toxic grain fumigant that can be produced from the reaction of metal phosphides with water. To determine the in vivo cytogenetic effects of inhalation of PH 3 , male CD‐1 mice were exposed to either 0, 5, 10, or 15 ppm target concentrations of PH 3 for 6 hr. Twenty hours after the termination of exposure, the spleens of the mice were removed, macerated, and the splenocytes cultured for analyses of sister chromatid exchanges, chromosome aberrations, and micronuclei in cytochalasin B‐in‐duced binucleated cells. In addition, bone marrow smears were made for the analysis of micronuclei in polychromatic erythrocytes. No increase in any of the cytogenetic endpoints was found at any of the concentrations examined. The only statistically significant response was a concentration‐related slowing of the cell cycle in the splenocytes. © 1994 Wiley‐Liss, Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.