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Predominant induction of kinetochore‐containing micronuclei by extracts of diesel exhaust particulates in cultured human lymphocytes
Author(s) -
Odagiri Youichi,
Zhang JianXin,
Uchida Hiroyuki,
Kawamura Ken,
Adachi Shuichi,
Takemoto Kazuo
Publication year - 1994
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850230108
Subject(s) - micronucleus test , micronucleus , chromosome aberration , aneuploidy , biology , microbiology and biotechnology , chemistry , chromosome , genetics , toxicity , gene , organic chemistry
Abstract The aneuploidy‐inducing activity of extracts of diesel exhaust particulates from light duty (LD) and heavy duty (HD) engines was investigated in cultured peripheral blood lymphocytes of 8 healthy donors using the cytokinesis‐block micronucleus test with the kinetochore labelling modification. A majority of the subjects tested showed a significant kinetochore‐positive micronucleus induction after treatment with the highest dose (150 μg/ml) of LD extract, although some subjects also showed induction of kinetochore‐negative micronuclei. Only one subject had significantly increased numbers of kinetochore‐positive micronuclei at a dose of 400 μg/ml of HD extract. These results suggest that diesel extract, at least LD extract, possesses the ability to induce whole chromosome loss (aneuploidy) preferentially, although there are also chromosome breaks. © 1994 Wiley‐Liss, Inc.