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Sources of variability in data from a lacl transgenic mouse mutation assay
Author(s) -
Piegorsch Walter W.,
Lockhart AnnMarie C.,
Margolin Barry H.,
Tindall Kenneth R.,
Gorelick Nancy J.,
Short Jay M.,
Carr Gregory J.,
Thompson Edward D.,
Shelby Michael D.
Publication year - 1994
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850230105
Subject(s) - transgene , mutation , biology , mutant , genetically modified mouse , escherichia coli , genetics , mutagenesis , microbiology and biotechnology , gene
Experimental features of a transgenic mouse mutation assay based on a lacl target transgene from Escherichia coli are considered in detail. Sources of variability in the experimental protocol that can affect the statistical nature of the observations are examined with the goal of identifying sources of excess variation in the observed mutant fractions. The sources include plate‐to‐plate (within packages), package‐to‐package (within animals), and animal‐to‐animal (within study) variability. Data from two laboratories are evaluated, using various statistical methods to identify excess variability. Results suggest only scattered patterns of excess variability, except possibly in those cases where genomic DNA from test animals is stored for extended periods (e.g., > 90 days) after isolation from tissues. Further study is encouraged to examine the validity and implications of this time/storage‐related effect.