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Investigation of aneuploidy induction in mouse oocytes following exposure to vinblastine‐sulfate, pyrimethamine, diethylstilbestrol diphosphate, or chloral hydrate
Author(s) -
Mailhes John B.,
Aardema Marilyn J.,
Marchetti Francesco
Publication year - 1993
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850220209
Subject(s) - chloral hydrate , aneuploidy , vinblastine , diethylstilbestrol , nondisjunction , andrology , biology , endocrinology , medicine , pharmacology , genetics , chromosome , chemotherapy , estrogen , gene
The various causative and mechanistic phenomena associated with aneuploidy induction require considerable investigation to better understand the etiology of chromosome missegregation. We investigated the potential of vinblastine sulfate, pyrimethamine, diethylstilbestrol diphosphate, and chloral hydrate to induce numerical and structural chromosome changes in female mouse germ cells. Superovulated ICR mice were administered the compounds either by intraperitoneal injection or oral gavage, and oocytes were collected and processed for cytogenetic analysis 17 hr later. Vinblastine sulfate, administered i.p., induced a significant increase in the frequency of ovulated Ml oocytes and of hyperploid Mll oocytes compared to controls, but did not increase the frequency of structural aberrations. Pyrimethamine, diethylstilbestrol diphosphate, and chloral hydrate did not increase the frequency of numerical or structural chromosome changes in female mouse germ cells. © 1993 Wiley‐Liss, Inc.