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Micronucleus test and erythropoiesis: Effect of cobalt on the induction of micronuclei by mutagens
Author(s) -
Suzuki Yuji,
Shimizu Hidesuke,
Nagae Yusuke,
Fukumoto Masakatsu,
Okonogi Hideo,
Kadokura Makoto
Publication year - 1993
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850220208
Subject(s) - micronucleus test , mutagen , erythropoiesis , cobalt , micronucleus , carcinogen , chemistry , pharmacology , bone marrow , biochemistry , toxicology , biology , medicine , toxicity , immunology , anemia , organic chemistry
The micronucleus test is used widely as an in vivo short‐term assay for potential carcinogens. In the present study, results of the micronucleus test were affected by cobalt dichloride pretreatment. Cobalt dichloride was used to induce erythropoietin, a growth factor for erythropoiesis. The increase in mutagen‐induced micronucleus response following cobalt pretreatment, therefore, may have been due to a change in the rate of erythropoiesis. The greatest interaction between cobalt pretreatment and mutagen treatment for the induction of micronucleated polychromatic erythrocytes (MPCE) occurred when mice were injected with 1,1‐dimethylhydrazine (DMH) 12–24 hr after pretreatment with cobalt dichloride and killed 30 hr later. Increased sensitivity of the micronucleus test was attributable to the administration of mutagen during the differentiation and multiplication of erythroblast, which is presumed to have been accelerated by pretreatment with cobalt dichloride. An increased induction of MPCE in the bone marrow by two chemicals—benzo(a)pyrene, 2‐naphthylamine—was also observed following pretreatment with cobalt dichloride. © 1993 Wiley‐Liss, Inc.