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Analysis of restriction enzyme‐induced chromosomal aberrations by fluorescence in situ hybridization
Author(s) -
Columna Elma Abella,
Giaccia Amato J.,
Evans James W.,
Yates Barbara L.,
Morgan William F.
Publication year - 1993
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850220106
Subject(s) - fluorescence in situ hybridization , in situ , biology , in situ hybridization , enzyme , genetics , restriction enzyme , microbiology and biotechnology , chemistry , biochemistry , dna , chromosome , gene , gene expression , organic chemistry
Fluorescence in situ hybridization and Giemsa staining of metaphase chromosomes were used to determine the relative frequencies of symmetric exchange aberrations (translocations) and asymmetric exchange aberrations (rings, dicentrics, and polycentrics) after exposure of human lymphoblastoid cells to restriction enzymes or X‐rays. The yield of symmetric exchanges was determined with the use of chromosome‐specific probes for human chromosomes 2 or 4, which were hybridized to metaphase chromosomes from cells exposed to the enzymes Pvull, Sacl, or Xbal or 3 or 5 Gy of X‐rays. The yield of asymmetric exchanges was determined in Giemsastained metaphase chromosomes from the same enzyme‐treated or irradiated cell population. About 1.5‐ to 3‐fold more symmetric than asymmetric exchanges were induced after restriction enzyme treatment. However, after X‐ray treatment the yield of dicentrics relative to the yield of reciprocal translocations was close to the expected 1:1 ratio.