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Modification of clastogenicity of three known clastogens by garlic extract in mice in vivo
Author(s) -
Das Tandra,
Roychoudhury Arati,
Sharma Archana,
Talukder Geeta
Publication year - 1993
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850210410
Subject(s) - clastogen , allium sativum , antimutagen , allicin , micronucleus test , in vivo , mutagen , biology , mitomycin c , bone marrow , genotoxicity , cyclophosphamide , toxicity , micronucleus , allium , sodium arsenite , pharmacology , toxicology , chemistry , food science , carcinogen , biochemistry , botany , genetics , chemotherapy , immunology , arsenic , organic chemistry
The anticlastogenic activity of crude extract of garlic ( Allium sativum L.) was studied in bone marrow cells of mice. Male laboratory‐bred Swiss albino mice were given one of three concentrations of the freshly prepared extract (100 mg, 50 mg, and 25 mg/kg body weight) as a dietary supplement by gavage for 6 consecutive days. On the seventh day the mice were administered a single acute dose of two known clastogens, mitomycin C(1.5 mg/kg) and cyclophosphamide (25 mg/kg) or sodium arsenite (2.5 mg/kg), simultaneously with garlic extract. After 24 hr, chromosome preparations were made from the bone marrow cells. The endpoint studied were chromosomal aberrations and damaged cells. Garlic extract alone induced a low level of chromosomal damage. The clastogenicity of all three mutagens were reduced significantly in the animals which had been given garlic extract as dietary supplement. The extent of reduction was different for the three clastogens and may be attributed to the interaction with the different components of the extract. © 1993 Wiley‐Liss, Inc.