Premium
Genotoxicity analysis of N,N‐dimethylaniline and N,N‐dimethyl‐p‐toluidine
Author(s) -
Taningher Maurizio,
Pasquini Rossana,
Bonatti Stefania
Publication year - 1993
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850210406
Subject(s) - genotoxicity , carcinogen , ames test , mutagenesis , clastogen , dna , in vivo , chromosome aberration , biology , microbiology and biotechnology , micronucleus , dna damage , toxicity , micronucleus test , chromatid , chromosome , toxicology , biochemistry , mutation , chemistry , genetics , gene , salmonella , organic chemistry , bacteria
N,N ‐Dimethylaniline (DMA, CAS No. 121—69—7) and N,N ‐dimethyl‐ p ‐toluidine (DMPT, CAS No. 99—97—8) belong to the N ‐dialkylaminoaromatics, a chemical class structurally alerting to DNA reactivity. Their applications may be industrial (dye and pesticide intermediates, polymerizing agents) and surgical (polymerization accelerators for the manufacture of bone cements and prosthetic devices), thus implying heterogeneous types of human exposure. Findings of carcinogenicity in rodents and some nonexhaustive genotoxicity data are available for DMA, but to our knowledge no information is available on DMPT concerning either carcinogenicity or any kind of genetic toxicity. To investigate their mechanism cf action and mutagenic/carcinogenic potential, DMA and DMPT were analyzed for complementary genotoxicity end‐points, namely, gene mutation in Salmonella (Ames test), structural and numerical chromosome aberrations in hamster V79 cells (micronucleus test, matched with an immunofluorescent staining for kinetochore proteins), and in vivo DNA damage in mouse and rat liver (alkaline DNA elution test). The results essentially indicate that both chemicals are chromosome damaging agents. Indeed, at the maximum nontoxic doses, they proved nonmutagenic in Salmonella (although their toxicity did not allow concentrations > 70 μg/plate to be tested) and weakly positive in inducing DNA damage (increases in DNA elution rates at most ∼2.4 times control value). Conversely, they proved clearly positive in inducing numerical chromosome alterations, with dose‐dependent increases up to more than five times the control value for DMPT. At the highest dose tested, both chemicals also showed a significant clastogenic effect. © 1993 Wiley‐Liss, Inc.