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Induction of micronuclei by bleomycin in G 0 human lymphocytes: I. Dose‐response and distribution
Author(s) -
Hoffmann George R.,
Colyer Shirley P.,
Littlefield L. Gayle
Publication year - 1993
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850210206
Subject(s) - micronucleus test , bleomycin , micronucleus , cytokinesis , biology , clastogen , microbiology and biotechnology , immunology , genetics , cell , chemistry , toxicity , cell division , chemotherapy , organic chemistry
The cytokinesis‐block micronucleus assay was used to investigate the induction of chromosomal damage by bleomycin in G 0 human lymphocytes. A dose‐dependent increase in the frequency of micronuclei was observed in binucleate cells, and the frequency approached 0.5 micronuclei per cell at the highest dosage tested. The distribution of micronuclei among cells was overdispersed, rather than fitting a Poisson distribution. Even at the highest dosage, more than two‐thirds of the cells did not contain micronuclei, while some cells were highly damaged, containing more than 4 micronuclei percell. © 1993 Wiley‐Liss, Inc.