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Mutagenicity of the human carcinogen treosulphan, and its hydrolysis product, dl‐1,2:3,4‐diepoxybutane in mammalian cells
Author(s) -
Zhu Songyun,
Zeiger Errol
Publication year - 1993
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850210113
Subject(s) - chinese hamster ovary cell , carcinogen , chemistry , cytotoxicity , hydrolysis , hamster , biochemistry , chinese hamster , cytotoxic t cell , mutagen , genotoxicity , microbiology and biotechnology , dna , stereochemistry , toxicity , biology , in vitro , organic chemistry , receptor
The cytotoxicity and mutagenicity of the human carcinogen, treosulphan, and its hydrolysis product, dl‐1,2:3,4‐diepoxybutane (DEB), were studied in Chinese hamster ovary, AS52, cells. Treosulphan (0.1–1.0 mM) is toxic and mutagenic at the gpt locus. A strong pH dependence was noted. DEB is cytotoxic and mutagenic at a much lower dose (0.025 mM), but these effects were not affected by pH. The results suggest that the toxic and mutagenic effects of treosulphan are mediated by its hydrolysis product DEB, and that the conversion of treosulphan to DEB is highly pH‐dependent. © 1993 Wiley‐Liss, Inc.