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Cytogenetic effects of vincristine sulfate and ethylene dibromide in human peripheral lymphocytes:Micronucleus Analysis
Author(s) -
Ong T.,
Nath J.
Publication year - 1992
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850200207
Subject(s) - micronucleus test , binucleated cells , micronucleus , biology , andrology , microbiology and biotechnology , immunology , toxicity , medicine
Microncuclei kinetics and persistence in mononucleated and binucleated human peripheral lymphocytes following short‐term (4 hr) and continuous (until harvest) in vitro exposure to vincristine sulfate (VS) and ethlylene dibrode (EDB) were studied. Lymphocytes were exposed to chemicals for various doses and harvested at different culture times. Micronucleus frequenvies were scored in both mononucleated and biucleated cells on the same slide. VS‐treated cells showed a significantly higher incidence of micronucleus in both ononucleated and binucleated cells than controls (p<0.01). The cells treated continuously with VS produced comparatively higher frequencies of micronucleated cells than those treated for 4 hr. Highest micronuclei frequencies were observed 24 hr after chemical treatment in both mononucleated and binucleated cells and decreased later with time. However, the micronucleus frequencies remained significantly higher than the controls even in the cells harvested at 144 hr. VS induced a large number of micronucleated cells with multiple micronuclei. VS also caused a severe decrease in nuclear division due to cytotoxic effect. Lymphoctes treated with EDB for 4 hr and continuously showed a statistically higher incidence of micronuclei in binucleated cells compared to the controls (p<0.05), whereas in mononucleated cells higher micronucleus frequencies were observed only in cultures treated continuously. Continuous presence of EDB induced both dose‐and time‐dependent increase of micronuclei in both mono‐ and binucleated cells(p<0.05). EDB induced relatively few multiple micronucleated cells in comparison with VS. EDB did not affect nuclear divisions even with continuous treatment. HIgh micronucleus frequencies observed at 144 hr harvest following 4 hr treatment of both EDB and VS suggest the persistence of DNA damage in cells. These studies suggest that micronuclei kinetics in human periphearl lymphocytes depends on the genotoxic potentially and cytotoxicity of a geno toxicant. © 1992 Wiley‐Liss, Inc.