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Mutagenicity of benzo(a)pyrenyl‐1‐sulfate in the Ames test
Author(s) -
Irwin Susan E.,
Kwei Gloria Y.,
Blackburn Gary R.,
Thurman Ronald,
Kauffman Fredrick C.
Publication year - 1992
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850190310
Subject(s) - chemistry , ames test , sulfate , arylsulfatase , benzo(a)pyrene , pyrene , sulfatase , biochemistry , carcinogen , stereochemistry , enzyme , salmonella , organic chemistry , biology , bacteria , genetics
Comparison of the mutagenicity of nine isomeric benzo(a)pyrenyl [B(a)P] phenols conjugated with either sulfate or glucuronide was carried out using strain Salmonella typhimurium TA98. Of the nine conjugates tested, only B(a)P‐1‐sulfate was mutagenic. Accordingly, the mutagenicity of B(a)P‐1‐sulfate was compared with that of B(a)P and 1‐hydroxybenzo(a)pyrene [B(a)P‐1‐OH] in the presence and absence of rat lung S9 and Aroclor‐induced liver S9 with and with out an NADPH‐generating system. B(a)P‐1‐sulfate was slightly mutagenic, whereas B(a)P and the 1‐hydroxy derivative were nonmutagenic when S9 fractions and NADPH were omitted. Addition of induced liver S9 with NADPH caused mutagenicity with B(a) ‐1‐OH>B(a)P>B(a)P‐1‐sulfate. B(a)P‐1‐sulfate was the only mutagenic species when lung S9 was added. This mutagenicity did not require NADPH. Sodium sulfite, an inhibitor of arylsulfatase, decreased the mutagenicity of B(a)P‐1‐sulfate. These data suggest that a unique mutagenic species is generated from B(a)P‐1‐sulfate via arylsulfatase in rat lung.