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Nitrobenzo[a]pyrene‐induced DNA amplification in SV40‐transformed chinese hamster embryo cells
Author(s) -
Neft Robin E.,
Roe Amy L.,
Schol Henry M.,
Fu Peter P.,
Mittelstaedt Roberta A.,
Casciano Daniel A.
Publication year - 1992
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850190210
Subject(s) - chinese hamster ovary cell , embryo , carcinogen , biology , hamster , dna , chinese hamster , carcinogenesis , microbiology and biotechnology , gene , cell culture , genetics
Nitrobenzo[a]pyrenes (NBaPs) are ubiquitous environmental pollutants that produce mulations in Salmonella typhimurium and Chinese hamster ovary cells. In this study, 1‐, 3‐, and 6‐NBaP induced amplification of SV40 DNA sequences in an SV40‐transformed Chinese hamster embryo cell line which is sensitive to DNA amplification by various known carcinogens. Of the three isomers, 3‐NBaP produced the highest level of gene amplification, which was 4.8 relative to untreated controls at a dose of 5 μg/ml. Considering the relationship between gene amplification and tumorigenesis, it seems prudent to carry out a more exhaustive analysis of the carcinogenic potential of these agents.