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Direct DNA sequencing of PCR products
Author(s) -
Zimmerman Lisa J.,
Fuscoe James C.
Publication year - 1991
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850180413
Subject(s) - primer dimer , polymerase chain reaction , primer (cosmetics) , dna sequencing , massive parallel sequencing , multiple displacement amplification , sequencing by ligation , biology , in silico pcr , applications of pcr , dna , inverse polymerase chain reaction , polymerase chain reaction optimization , hot start pcr , computational biology , genetics , microbiology and biotechnology , digital polymerase chain reaction , multiplex polymerase chain reaction , chemistry , base sequence , dna extraction , gene , genomic library , organic chemistry
Experiments are described which elucidate some of the technical problems associated with the direct sequencing of polymerase chain reaction (PCR) amplified DNA. Sequencing primer purity, labeling methodology, and template preparation were explored. Conditions are presented for the routine sequencing of single‐ and double‐stranded PCR products.