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DNA sequence mapping by fluorescence in situ hybridization
Author(s) -
Brandriff B. F.,
Gordon L. A.,
Trask B. J.
Publication year - 1991
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850180410
Subject(s) - chromatin , fluorescence in situ hybridization , biology , dna , metaphase , in situ , heteroduplex , in situ hybridization , hybridization probe , dna sequencing , microbiology and biotechnology , chromosome , fluorescence microscope , genetics , computational biology , fluorescence , chemistry , gene , gene expression , physics , organic chemistry , quantum mechanics
Various types of DNA probes, such as total genomic DNA, repetitive sequences, unique sequences, and composites of chromosome‐specific DNA probes, can be used with fluorescence in situ hybridization (FISH) techniques to address research questions having to do with localization, mapping, and distribution of DNA in situ. FISH involves the formation of a heteroduplex between such DNA probes and chromatin targets on a microscope slide, which can be visualized with fluorescent reporter molecules. Three chromatin targets—metaphase chromosomes, somatic interphases, and zygote interphases‐offer increasingly extended states of chromatin which can be strategically selected, individually or in combination, to address specific research questions of interest.