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Workshop overview: New molecular techniques in genome analysis
Author(s) -
DeMarini David M.,
Fuscoe James C.
Publication year - 1991
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850180403
Subject(s) - biology , southern blot , pulsed field gel electrophoresis , genomic dna , gel electrophoresis , temperature gradient gel electrophoresis , polymerase chain reaction , multiplex polymerase chain reaction , microbiology and biotechnology , nucleic acid thermodynamics , comparative genomic hybridization , genetics , fluorescence in situ hybridization , restriction enzyme , genome , dna , gene , base sequence , chromosome , 16s ribosomal rna , genotype
An overview of the application of various molecular techniques to the analysis of genomic DNA is presented. For the analysis of small‐scale changes, the polymerase chain reaction (PCR), colony probe hybridization, mismatch hybridization, and denaturing gradient gel electrophoresis (DGGE) are providing information on mutations within prokaryotic and eukaryotic genes. For large‐scale changes, fluorescence in situ hybridization (FISH), pulsed‐field gel electrophoresis (PFGE), Southern blotting, multiplex PCR, hybridization of linked probes, and restriction enzyme mapping are permitting analysis of genomic alterations that are larger than point mutations but below the resolution of standard cytogenetic analysis. Many of these techniques, either alone or in combination, produce DNA that can be subjected to DNA sequence analysis, which provides the most detailed information regarding genomic changes.