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Enumeration of 6‐thioguanine‐resistant T‐lymphocytes in the peripheral blood of nonhuman primates (cynomolgus monkeys)
Author(s) -
Zimmer D. M.,
Aaron C. S.,
O'Neill J. P.,
Albertini R. J.,
Carver J. H.,
Hoffman G. R.
Publication year - 1991
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850180304
Subject(s) - ethylnitrosourea , biology , microbiology and biotechnology , phenotype , somatic cell , mutagenesis , peripheral blood , mutation frequency , immunology , mutation , andrology , genetics , medicine , gene , mutant
We have investigated the use of cynomolgus monkeys ( Macaca fascicularis ) as a model of somatic cell mutagenesis in non‐human primates. Using techniques described by Albertini ( Mutation Research 150:411–422, 1985) for similar studies in humans, the frequency of TG‐resistant T‐lymphocytes in the peripheral blood was determined in animals that were either untreated or treated with ethylnitrosourea. The frequency of TG‐resistant cells in untreated males was (mean ± 5D) 6.0 ± 5.9 per 10 6 cells and for untreated females was 2.9 ± 2.7 per 10 6 cells. The spontaneous frequency of TG‐resistant cells for all animals was 4.2 ± 4.44 per 10 6 cells. Maximum frequency of TG‐resistant cells for two animals treated with a single I.P. dose of ENU was 45.1 and 77.9 per 10 6 cells. Substantial increases in frequencies of TG‐resistant cells were not seen until at least 63 days after treatment. The TG‐resistant phenotype of clones isolated in the assay was stable after growth for 2 weeks in the absence of selective agent. Many of the TG‐resistant clones selected were frozen for future molecular analysis.