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Replication index in cultured human lymphocytes: Methods for statistical analysis and possible role in genetic toxicology
Author(s) -
Lazutka J. R.,
Margolin B. H.
Publication year - 1991
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850170308
Subject(s) - replication (statistics) , replicate , genotoxicity , statistical analysis , index (typography) , type i and type ii errors , toxicology , statistics , biology , statistical power , computational biology , monte carlo method , genetics , computer science , mathematics , toxicity , chemistry , organic chemistry , world wide web
The replication index (RI) is an index of cytokinetics in cultured cells. This paper presents statistical methods for the analysis of RI data. It also proposes formulas for the calculation of RI and its standard error. The proposed statistical analysis may be applied only in cases when replicate cultures are used. If duplicate or repeated cultures are used, the interculture variation may provide a more suitable error term for RI. Determining the RI in human blood cultures may be useful in genotoxicity testing, because inhibition of DNA synthesis will result in a decrease of the RI. Monte Carlo methods are used to show that the sensivity of the proposed method is roughly equivalent to that of a χ 2 test. Power studies indicate that for the study sample, no less than 200 cells should be analyzed when assessing the RI.