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Host cell reactivation of sunlamp‐exposed adenovirus in fibroblasts from patients with bloom's syndrome, ataxia telangiectasia, and huntington's disease
Author(s) -
Rainbow Andrew J.,
Skopek T. R.
Publication year - 1991
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850170206
Subject(s) - xeroderma pigmentosum , bloom syndrome , ataxia telangiectasia , fibroblast , biology , virology , dna , dna repair , genetics , dna damage , cell culture , rna , helicase , gene
In this study, a sensitive host cell reactivation (HCR) technique was used to examine the repair capacity for DNA damaged by sunlamp exposure in fibroblast strains derived from 5 normal individuals and 8 patients representing three different diseases associated with DNA repair deficiencies. Adenovirus type 2 (Ad 2) was exposed to radiation from a GE 275 W sunlamp and subsequently used to infect fibroblast monolayers. At 48 hr after infection, cells were scored for the presence of viral structural antigens (Vag) using indirect immunofluorescent staining. Previous reports using this technique showed a substantial reduction in the HCR of sunlamp‐exposed Ad 2 for infection of excision repair deficient fibroblasts from patients with xeroderma pigmentosum. In contrast, the HCR of Vag synthesis for sunlamp‐exposed Ad 2 was in the normal range for the three ataxia telan‐giectasia, three Bloom's syndrome, and two Huntington's disease fibroblast strains.