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The simultest approach for testing mutagens in the Salmonella Microtitre fluctuation assay
Author(s) -
McPherson Marcia F.,
Nestmann Earle R.,
Hartman P. E.
Publication year - 1990
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850160104
Subject(s) - salmonella , ames test , screening test , chemistry , food science , chromatography , toxicology , biology , bacteria , genetics , medicine , family medicine
Abstract The concept of combining several histidine‐dependent Salmonella strains in a single test, the SIMULTEST, has been applied to the microtitre fluctuation test. The activity of five mutagens was determined in strains TA97, TA98, TA100, and TA102 individually as well as in a SIMULTEST mixture. All five compounds were mutagenic in the SIMULTEST, demonstrating the utility of this time and labour‐saving approach of combining strains for testing with this method. The microtitre fluctuation SIMULTEST results were quantitatively comparable to those of the SIMULTEST Salmonella /microsome plate test. The microtitre fluctuation test compared with the plate incorporation assay generally showed more favourable “sensitivity” and “quantity” indices in that four of the five chemicals tested in the fluctuation test were mutagenic at lower doses than in the plate test.