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Effect of interleukin‐2 on cell proliferation, sister‐chromatid exchange induction, and nuclear stress protein phosphorylation in Pha‐stimulated fischer 344 rat spleen lymphocytes: Modulation by 2‐mercaptoethanol
Author(s) -
Morris Suzanne M.,
Aidoo Anane,
Domon Olen E.,
McGarrity Lynda J.,
Kodell Ralph L.,
Schol Henry M.,
Hinson William G.,
Pipkin James L.,
Casciano Daniel A.
Publication year - 1990
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850150103
Subject(s) - sister chromatid exchange , microbiology and biotechnology , biology , phosphorylation , cell growth , mitotic index , interleukin 2 , sister chromatids , interleukin , biochemistry , mitosis , in vitro , immunology , cytokine , gene , chromosome
The effect of interleukin‐2 (IL‐2) on cell proliferation, sister‐chromatid exchange (SCE) frequency, and the phosphorylation of nuclear stress proteins was evaluated in phytohemagglutinin (PHA)‐stimulated spleen lymphocytes isolated from Fischer 344 rats. In addition, the ability of 2‐mercaptoethanol (2‐ME) to modulate the induction of these biological responses was characterized. Cell proliferation, as measured by the mitotic index, increased significantly ( P < .003) from a range of 3‐4% in PHA‐stimulated cultures to a range of 8‐11% in PHA‐stimulated cultures exposed to IL‐2. The average generation time (AGT) did not respond to IL‐2 in a concentration‐dependent manner and decreased significantly ( P < .05) when 20 μM 2‐ME was includedwith IL‐2 in the culture medium. The number of SCE increased significantly ( P < .004) from control frequencies, which ranged from 13.1 to 15.6 SCE per cell, to frequencies of 18.5 to 21.5 SCE per cell as the concentration of IL‐2 in the culture medium increased to 50 half‐maximal units per ml. A reduction in SCE frequency was observed when cells were cultured with 20 μM 2‐ME and IL‐2 compared to IL‐2 alone. Three nuclear proteins, with relative molecular masses of approximately 13,000‐18,000, 20,000, and 80,000, were phosphorylated in IL‐2‐exposed G 1 ‐phase nuclei. Elicitation of these nuclear proteins in IL‐2‐exposed cells was not affected by exposure to 2‐ME.