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Sister chromatid exchanges induced by tertiary butyl hydroquinone in bone marrow cells of mice
Author(s) -
Mukherjee Anita,
Talukder Geeta,
Sharma Archana
Publication year - 1989
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850130307
Subject(s) - hydroquinone , sister chromatids , sister chromatid exchange , bone marrow , chemistry , metaphase , mutagen , cell cycle , in vivo , antioxidant , dna damage , apoptosis , toxicology , microbiology and biotechnology , andrology , biochemistry , biology , dna , genetics , immunology , medicine , chromosome , gene
Tertiary butyl hydroquinone (TBHQ)—a phenolic antioxidant, was evaluated by assessing the induction of sister chromatid exchanges (SCEs) in bone marrow metaphase cells of mice. Six concentrations, 0.5, 2, 20, 50, 100, and 200 mg/kg, of TBHQ were injected intraperitoneally. A positive dose‐response effect in the SCE frequency was observed using the Cochran‐Armitage trend test. Two mg/kg of TBHQ was found to be the minimum effective dose. Study of the cell‐cycle kinetics showed a delay in cell cycle induced by the higher concentrations of TBHQ. Thus, TBHQ was found to be a DNA damage‐inducing agent and also a cytotoxic chemical in vivo in mice.