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Sodium thiosulfate inhibits cisplatin induced mutagenesis in somatic tissue of Drosophila
Author(s) -
Katz Alan J.
Publication year - 1989
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850130202
Subject(s) - cisplatin , sodium thiosulfate , mutagenesis , genotoxicity , drosophila melanogaster , chemistry , mutagen , somatic cell , mitotic crossover , microbiology and biotechnology , biology , toxicity , biochemistry , toxicology , carcinogen , mutation , genetics , gene , inorganic chemistry , organic chemistry , chemotherapy
The antitumor agent cisplatin was evaluated for genotoxicity in the somatic tissue of Drosophila melanogaster in combination with the nucleo‐philic compound sodium thiosulfate (STS). Third instar larvae transheterozygous for mwh and flr 3 were grown on media containing (1) water, (2) 200 mM STS, (3) 0.05 mM cisplatin in 200 mM STS, or (4) 0.05 mM cisplatin. Wings of surviving adults were scored for the presence of twin spots and both small and large single spots; 200 mM STS was nongenotoxic in the assay. Whereas 0.05 mM cisplatin significantly induced all three endpoints, 0.05 mM cisplatin in combination with 200 mM STS was found to have no genotoxic activity. Hence, STS possessed antimutagenic activity in the wing‐spot assay and completely inhibited cisplatin‐induced mutagenesis and mitotic recombination.