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Effect of treatment medium on induction of aneuploidy by nocodazole in saccharomyces cerevisiae
Author(s) -
TaylorMayer Rhoda E.,
Mayer Ver W.,
Goin Carol J.
Publication year - 1988
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850110305
Subject(s) - nocodazole , saccharomyces cerevisiae , aneuploidy , benomyl , yeast , acetone , chemistry , carbamate , strain (injury) , biology , biochemistry , botany , gene , cell , fungicide , chromosome , anatomy , cytoskeleton
Abstract While studying ways to improve responsiveness of Saccharomyces cerevisiae strain D61.M to agents that induce aneuploidy, we noted that nocodazole, which strongly induces aneuploidy when yeast cells are treated in yeast extract‐peptone‐dextrose (YEPD) medium, had no effect when a synthetic complete (SC) medium was used. Further study revealed that the presence of peptone was necessary for induction. Other aneuploidy‐inducing agents, including ethyl acetate, acetone, and methyl benzimi‐dazoie‐2‐yl‐carbamate (MBC), were equally active in either medium. Benomyl, which degrades to MBC, was less active in SC than in YEPD medium.