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Genotoxicity of acrylic acid, methyl acrylate, ethyl acrylate, methyl methacrylate, and ethyl methacrylate in l5178y mouse lymphoma cells
Author(s) -
Moore Martha M.,
Amtower Amanda,
Doerr Carolyn L.,
Brock Karen H.,
Dearfield Kerry L.
Publication year - 1988
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850110107
Subject(s) - methacrylate , methyl methacrylate , acrylate , ethyl acrylate , chemistry , acrylic acid , mutant , methyl acrylate , microbiology and biotechnology , nuclear chemistry , monomer , biology , biochemistry , organic chemistry , polymer , gene
A series of monomeric acrylate/methacrylate esters (methyl acrylate, ethyl acrylate, methyl methacrylate, and ethyl methacrylate) as well as acrylic acid were examined for genotoxic activity in L5178Y mouse lymphoma cells without exogenous activation. All five compounds induced concentration‐dependent increases in mutant frequency. Small‐colony, trifluorothymidine‐resistant mutants were primarily induced, which suggests that these compounds may act via a clastogenic mechanism. This prediction was confirmed by the finding that all five compounds produced gross chromosome aberrations in mouse lymphoma cells. The two acrylates were much more potent in their response than acrylic acid. Methyl acrylate (22 μg/ml, survival = 18%) induced 385 mutants/10 6 survivors (total mutant frequency less the spontaneous mutant frequency) and 45 chromosome aberrations/100 cells analyzed (total aberrations less the spontaneous background). Ethyl acrylate (37.5 μg/ml, survival = 15%) induced 683 mutants/10 6 survivors.