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The simultest: A new approach to screening chemicals with the Salmonella reversion assay
Author(s) -
Nestmann Earle R.,
Brillinger Renata L.,
McPherson Marcia F.,
Maus K. Laurie
Publication year - 1987
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/em.2850100207
Subject(s) - salmonella , reversion , ames test , plasmid , strain (injury) , enterobacteriaceae , chemistry , biology , food science , microbiology and biotechnology , toxicology , genetics , bacteria , biochemistry , escherichia coli , dna , gene , phenotype , anatomy
A new Salmonella mutagenicity test method is under development to test a chemical with more than one strain simultaneously (the “SIMULTEST”), that is, different Salmonella typhimurium tester strains are used in combination on the same plate. Strains are combined in two sets: strains with plasmid pKM101 (TA97, TA98, TA100, and TA102) and strains without the plasmid (TA1535, TA1537, and TA1538). The SIMULTEST combinations successfully detect the mutagenic activity of five mutagens in different chemical classes. This approach may be useful in reducing the workload associated with mutagenicity testing with Salmonella .